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New capabilities for Mycosphaerella graminicola research

Tipo de material: TextoTextoSeries ; Molecular Plant Pathology, 7(5), p.691-704, 2005Trabajos contenidos:
  • Bowler, J
  • Scott, E
  • Tailor, R
  • Scalliet, G
  • Ray, J
  • Csukai, M
Recursos en línea: Resumen: Mycosphaerella graminicola is a major pathogen of wheat worldwide, causing Septoria leaf blotch disease. Targeted gene disruption in M. graminicola, by Agrobacterium tumefaciensmediated transformation, has become an established functional genomics tool for M. graminicola research in recent years. However, in order to advance research into this economically important pathogen, further functional genomics tools need to be developed. Here, we report three new capabilities for M. graminicola research: (i)two selectable markers have been shown to work robustly in M. graminicola, namely G418 and the fungicide carboxin; (ii)the generation of a strain of M. raminicola in which the KU70 (MUS-51)homologue has been disrupted; in this strain, homologous recombination efficiencies increased to more than 95
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Mycosphaerella graminicola is a major pathogen of wheat worldwide, causing Septoria leaf blotch disease. Targeted gene disruption in M. graminicola, by Agrobacterium tumefaciensmediated transformation, has become an established functional genomics tool for M. graminicola research in recent years. However, in order to advance research into this economically important pathogen, further functional genomics tools need to be developed. Here, we report three new capabilities for M. graminicola research: (i)two selectable markers have been shown to work robustly in M. graminicola, namely G418 and the fungicide carboxin; (ii)the generation of a strain of M. raminicola in which the KU70 (MUS-51)homologue has been disrupted; in this strain, homologous recombination efficiencies increased to more than 95

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