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Analysis of 34ndolylacetic Acid and Abscisic Acid by High-Performance Liquid Chromatography and Gas-Liquid Chromatography

Tipo de material: TextoTextoSeries ; Analytical BioChemistry, 146(1), p.184-190, 1985Trabajos contenidos:
  • Rodriguez, A
  • Sanchez Tames, R
Recursos en línea: Resumen: A method of analysis of 3-indolylacetic acid ([AA)and abscisic acid (ABA), allowing the simultaneous extraction of both regulators from plant material, has been developed. The method involves extraction with methanol, isolation of the acid fraction, diazomethane methylation, separation of the hormones through reverse-phase preparative high-performance liquid chromatography, and quantification of both compounds by gas-liquid chromatography. The recovery percentage at each step was monitored with radioactive compounds added at the beginning of the process. The final recovery was 70
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-13455 (Browse shelf(Opens below)) Available

A method of analysis of 3-indolylacetic acid ([AA)and abscisic acid (ABA), allowing the simultaneous extraction of both regulators from plant material, has been developed. The method involves extraction with methanol, isolation of the acid fraction, diazomethane methylation, separation of the hormones through reverse-phase preparative high-performance liquid chromatography, and quantification of both compounds by gas-liquid chromatography. The recovery percentage at each step was monitored with radioactive compounds added at the beginning of the process. The final recovery was 70

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