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DNA Isolation from Mammalian Samples

Tipo de material: TextoTextoSeries ; Current Protocols in Molecular Biology, 102(1), p.2-14, 2013Trabajos contenidos:
  • Liu, X
  • Harada, S
Tema(s): Recursos en línea: Resumen: DNA isolation is a fundamental initial step for molecular genetic studies. Two quite dif-ferent methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE)tissue samples.
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DNA isolation is a fundamental initial step for molecular genetic studies. Two quite dif-ferent methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE)tissue samples.

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