Image from Google Jackets

One-step purification of an avocado peroxidase

Tipo de material: TextoTextoSeries ; Plant Physiology and BioChemistry, 33(5), p.531-537, 1995Trabajos contenidos:
  • Sanchez Romero, C
  • Garcia Gomez, L
  • Pliego Alfaro, F
  • Heredia, A
Tema(s): Recursos en línea: Resumen: A rapid isolati on procedure was developed for purification of a soluble ani onic isoperoxidase (donor: hydrogen peroxide oxidoreductase, EC 1.11.1.7)from avocado (Persea all1ericana, M. cv. Topa Topa)leaves. Electroelution from a native polyacrylamide gel, was the only requirement to achieve this. The purified isoperox idase was a monomer, 60 illa molecular mass, with a carbohydrate moiety. The amino acid composition showed 358 amino acid residues and appeared to be similar to those reported in other plant peroxidases. The study of some enzymatic properties, e. g. substrate specificity, susceptibility to effectors and so on, revealed that this isoperoxidase follows, in general, the action patterns previously established for anionic isoperoxidases.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Status Date due Barcode
Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-17163 (Browse shelf(Opens below)) Available

A rapid isolati on procedure was developed for purification of a soluble ani onic isoperoxidase (donor: hydrogen peroxide oxidoreductase, EC 1.11.1.7)from avocado (Persea all1ericana, M. cv. Topa Topa)leaves. Electroelution from a native polyacrylamide gel, was the only requirement to achieve this. The purified isoperox idase was a monomer, 60 illa molecular mass, with a carbohydrate moiety. The amino acid composition showed 358 amino acid residues and appeared to be similar to those reported in other plant peroxidases. The study of some enzymatic properties, e. g. substrate specificity, susceptibility to effectors and so on, revealed that this isoperoxidase follows, in general, the action patterns previously established for anionic isoperoxidases.

There are no comments on this title.

to post a comment.