BITR bioreactor increases in vitro multiplication of quality shoots in sugarcane (Saccharum spp. variety LAICA 04.809)

The multiplication of sugarcane (Saccharum spp.)variety LAICA 04-809 by direct organogenesis was evaluated using four cultivation systems: (1)the traditional system which uses a semisolid medium and (2)three temporary immersion systems with liquid medium (RITA®, BIT®, and SETIST). In addition, three planting densities (10, 30, and 50 mL of medium per explant)were studied for each system as initial inoculum. Sucrose consumption was observed in all temporary immersion systems after 4 weeks of culture. Photomixotrophic metabolism was identified in all three temporary immersion systems evaluated (RITA®, BIT®, and SETIST). In addition, at density of 30 mL medium/explant, the BIT® bioreactor generated the highest number of quality shoots (425.33?±?24.58). No hyperhydricity was observed in the explants. In TISs, the 50 mL volume of medium per explant obtained the highest multiplication coefficient (RITA® 25.53?±?11.42, BIT® 20.08?±?4.1 and SETIST 11.47?±?1.27). The results of this study suggest that the combination of the BIT® system with the 50 mL volume of medium/explant represents the best conditions for mass propagation of sugarcane shoots after four weeks of cultivation.