Production of pineapple transgenic plants assisted by temporary immersion bioreactors
Production of pineapple transgenic plants assisted by temporary immersion bioreactors
- Plant Cell Reports, 21(2), p.136-140, 2002 .
Abstract. A procedure for producing pineapple [Ananas comosus (L.)Merr.]transgenic plants was developed that involved selection by micropropagation in temporary immersion bioreactors (TIBs). Pineapple calluses ranging in size from 1.5 mm to 2.0 mm that were co-cultivated with Agrobacterium tumefaciens strains AT2260 (pIG121Hm)and LBA4404 (pTOK233)for 24 h produced the highest percentage (40
TRANSGENIC PINEAPPLE
TEMPORARY IMMERSION BIOREACTOR
AGROBACTERIUM TUMEFACIENS
Abstract. A procedure for producing pineapple [Ananas comosus (L.)Merr.]transgenic plants was developed that involved selection by micropropagation in temporary immersion bioreactors (TIBs). Pineapple calluses ranging in size from 1.5 mm to 2.0 mm that were co-cultivated with Agrobacterium tumefaciens strains AT2260 (pIG121Hm)and LBA4404 (pTOK233)for 24 h produced the highest percentage (40
TRANSGENIC PINEAPPLE
TEMPORARY IMMERSION BIOREACTOR
AGROBACTERIUM TUMEFACIENS