Functional analysis of the calcineurin-encoding gene cnaA from Aspergillus oryzae: evidence for its putative role in stress adaptation
Functional analysis of the calcineurin-encoding gene cnaA from Aspergillus oryzae: evidence for its putative role in stress adaptation
- Archives of MicroBiology, 179(6), p.416-422, 2003 .
The presence of putative STRE (stress response regulatory element)and HSF (heat-shock factor)transcription factor binding sites in the promoter region of the gene encoding calcineurin (cnaA)from Aspergillus oryzae implicated a probable role for calcineurin in the stress response. The activity of calcineurin was enhanced during growth of the wild-type strain in the presence of 1 M NaCl (2.6-fold), at alkaline pH 10.0 (2.9-fold)and at 37 °C (1.6-fold). The induction of cnaA antisense expression resulted in reduced calcineurin activity (1.4-fold)and caused a growth defect under the stress conditions. Induction of a strain overexpressing cnaA resulted in an increase in calcineurin activity under stress conditions, such as the presence of 1 M NaCl (73
ASPERGILLUS ORYZAE
STRESS
CALCINEURIN A
CNAA
PROTEIN PHOSPHATASE 2B
CALMODULIN- DEPENDENT PROTEIN PHOSPHATASE
The presence of putative STRE (stress response regulatory element)and HSF (heat-shock factor)transcription factor binding sites in the promoter region of the gene encoding calcineurin (cnaA)from Aspergillus oryzae implicated a probable role for calcineurin in the stress response. The activity of calcineurin was enhanced during growth of the wild-type strain in the presence of 1 M NaCl (2.6-fold), at alkaline pH 10.0 (2.9-fold)and at 37 °C (1.6-fold). The induction of cnaA antisense expression resulted in reduced calcineurin activity (1.4-fold)and caused a growth defect under the stress conditions. Induction of a strain overexpressing cnaA resulted in an increase in calcineurin activity under stress conditions, such as the presence of 1 M NaCl (73
ASPERGILLUS ORYZAE
STRESS
CALCINEURIN A
CNAA
PROTEIN PHOSPHATASE 2B
CALMODULIN- DEPENDENT PROTEIN PHOSPHATASE