Isolation of mutants of unable to synthesize cadystin, small cadmium-binding peptides
Isolation of mutants of unable to synthesize cadystin, small cadmium-binding peptides
- BioChemical and Biophysical Research Communications , 151(1), p.32-39, 1988 .
synthesize small cadmium-binding peptides cadystin, structure of which is (-Glu-Cys)n-Gly, in response to cadmium. Mutants unable to synthesize cadystin were found in the mutants hypersensitive to cadmium. Some of them lack activity of either -glutamylcysteine synthetase (EC 6.3.2.2)or glutathione synthetase (EC 6.3.2.3), enzyme involved in glutathione biosynthesis. Some mutants have the same activity levels of these enzymes as wild type has. These results indicate that some steps of cadystin biosynthesis are catalyzed by the enzymes catalyzing glutathione biosynthesis.
EMS
ETHYLMETHANESULFONATE
DTT
DITHIOTHREITOL
synthesize small cadmium-binding peptides cadystin, structure of which is (-Glu-Cys)n-Gly, in response to cadmium. Mutants unable to synthesize cadystin were found in the mutants hypersensitive to cadmium. Some of them lack activity of either -glutamylcysteine synthetase (EC 6.3.2.2)or glutathione synthetase (EC 6.3.2.3), enzyme involved in glutathione biosynthesis. Some mutants have the same activity levels of these enzymes as wild type has. These results indicate that some steps of cadystin biosynthesis are catalyzed by the enzymes catalyzing glutathione biosynthesis.
EMS
ETHYLMETHANESULFONATE
DTT
DITHIOTHREITOL