A New Technique for Coconut (Cocos nucifera)Germplasm Collection from Remote Sites: Culturability of Embryos Following Low-temperature Incubation
A New Technique for Coconut (Cocos nucifera)Germplasm Collection from Remote Sites: Culturability of Embryos Following Low-temperature Incubation
- Australian Journal of Botany, 47(1), p.69-75, 1999 .
A new technique for coconut (Cocos nucifera L.)germplasm collection was evaluated in the laboratory and tested in the field in Indonesia. The technique involved the non-sterile isolation of embryos, and incubation in sterile ascorbic acid solution (1 mg L.1)at 5 ± 1°C in the dark. During this incubation period the embryos could be transported and/or stored for a period of up to 4 days without embryo viability loss. Following this period the embryos were surface sterilised with sodium hypochlorite (1.5
A new technique for coconut (Cocos nucifera L.)germplasm collection was evaluated in the laboratory and tested in the field in Indonesia. The technique involved the non-sterile isolation of embryos, and incubation in sterile ascorbic acid solution (1 mg L.1)at 5 ± 1°C in the dark. During this incubation period the embryos could be transported and/or stored for a period of up to 4 days without embryo viability loss. Following this period the embryos were surface sterilised with sodium hypochlorite (1.5