Line-Scan Raman Microspectrometry for Biological Applications
Line-Scan Raman Microspectrometry for Biological Applications
- Applied Spectroscopy, 51, p.1607-1612, 1997 .
developed for the study of biological samples such as cells and chromosomes. With the illumination of a line on the sample, all the spectral information of the line is recorded. The line-scan microscope was attained by the introduction of one scanning mirror, a slit, and two cylindrical lenses in a standard confocal Raman microscope. The resolu tion, for an ideal sample, is 0.5 m m in the lateral direction and 3.5 m m in the axial direction. Line-scan experiments were performed to demonstrate the applicability of the instrument for biological samples. Line-scan images of calcium phosphate bone implant coatings and of the banding pattern of polytene chromosomes are presented.
RAMAN
MICROSCOPY
RESOLUTION
LINE ILLUMINATION
developed for the study of biological samples such as cells and chromosomes. With the illumination of a line on the sample, all the spectral information of the line is recorded. The line-scan microscope was attained by the introduction of one scanning mirror, a slit, and two cylindrical lenses in a standard confocal Raman microscope. The resolu tion, for an ideal sample, is 0.5 m m in the lateral direction and 3.5 m m in the axial direction. Line-scan experiments were performed to demonstrate the applicability of the instrument for biological samples. Line-scan images of calcium phosphate bone implant coatings and of the banding pattern of polytene chromosomes are presented.
RAMAN
MICROSCOPY
RESOLUTION
LINE ILLUMINATION