Identification of genes expressed during spore germination of Mycosphaerella pinodes
Identification of genes expressed during spore germination of Mycosphaerella pinodes
- Journal of General Plant Pathology, 71(3), p.190-195, 2005 .
Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH)was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95
MYCOSPHAERELLA PINODES
PISUM SATIVUM
PYCNOSPORE GERMINATION
RT-PCR
SUPPRESSION SUBTRACTIVE HYBRIDIZATION (SSH)
Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH)was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95
MYCOSPHAERELLA PINODES
PISUM SATIVUM
PYCNOSPORE GERMINATION
RT-PCR
SUPPRESSION SUBTRACTIVE HYBRIDIZATION (SSH)