Isolation of a new dual-functional ca¡eine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to ca¡eine from coffee (Coffea arabica L.)1
Isolation of a new dual-functional ca¡eine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to ca¡eine from coffee (Coffea arabica L.)1
- Febs Letters, 534, p.75-81, 2003 .
In co¡ee and tea plants, ca¡eine is synthesized from xanthosine via a pathway that includes three methylation steps. We report the isolation of a bifunctional co¡ee ca¡eine synthase (CCS1)clone from co¡ee endosperm by reverse transcriptionpolymeras chain reaction (RT-PCR)and rapid ampli¢cation of cDNA ends (RACE)technique using previously reported sequence information for theobromine synthases (CTSs). The predicted amino acid sequences of CCS1are more than 80
CO¡EE
CA¡EINE
N-METHYLTRANSFERASE
7-METHYLXANTHINE
S-ADENOSYL-L-METHIONINE
THEOBROMINE
In co¡ee and tea plants, ca¡eine is synthesized from xanthosine via a pathway that includes three methylation steps. We report the isolation of a bifunctional co¡ee ca¡eine synthase (CCS1)clone from co¡ee endosperm by reverse transcriptionpolymeras chain reaction (RT-PCR)and rapid ampli¢cation of cDNA ends (RACE)technique using previously reported sequence information for theobromine synthases (CTSs). The predicted amino acid sequences of CCS1are more than 80
CO¡EE
CA¡EINE
N-METHYLTRANSFERASE
7-METHYLXANTHINE
S-ADENOSYL-L-METHIONINE
THEOBROMINE