Isolation of RNA and DNA fragments using diatomaceous earth1
Isolation of RNA and DNA fragments using diatomaceous earth1
- Biotechnology Techniques, 12(7), p.549-552, 1998 .
A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed. Cytoplasmic RNA isolated from hybridoma cells by this method was used in RTPCR. DNA fragments obtained using this protocol were suitable for further subcloning, gene transformation and DNA sequencing.
A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed. Cytoplasmic RNA isolated from hybridoma cells by this method was used in RTPCR. DNA fragments obtained using this protocol were suitable for further subcloning, gene transformation and DNA sequencing.