Micropropagation of Venus fly trap by shoot culture
Micropropagation of Venus fly trap by shoot culture
- Plant Cell, Tissue and Organ Culture, 72, p.95-98, 2003 .
In order to establish and optimize an in vitro micropropagation protocol of Venus fly trap (Dionaea muscipula Ellis), a carnivorous plant, the effects of medium type, MS medium concentration, pH, and cytokinin and auxin types on shoot proliferation and root formationwere investigated using 3-month-old shoots. The shoot proliferation was most effective in 2.3 ìM kinetin-supplemented 1/3MS medium at pH 5.5. The best conditions for rooting were 1/3MS medium supplemented with 0.5 ìM IBA. All subcultured shoots produced extensive root systems after 5-6 weeks culture. When plantlets after rooting were planted in plastic pots filled with 1:1 peat moss and sand, the survival rate of plantlets was almost 100
DIONAEA MUSCIPULA ELLIS
VENUS FLY TRAP
SHOOT CULTURE
MS MEDIUM
KINETIN
MICROPROPAGATION
In order to establish and optimize an in vitro micropropagation protocol of Venus fly trap (Dionaea muscipula Ellis), a carnivorous plant, the effects of medium type, MS medium concentration, pH, and cytokinin and auxin types on shoot proliferation and root formationwere investigated using 3-month-old shoots. The shoot proliferation was most effective in 2.3 ìM kinetin-supplemented 1/3MS medium at pH 5.5. The best conditions for rooting were 1/3MS medium supplemented with 0.5 ìM IBA. All subcultured shoots produced extensive root systems after 5-6 weeks culture. When plantlets after rooting were planted in plastic pots filled with 1:1 peat moss and sand, the survival rate of plantlets was almost 100
DIONAEA MUSCIPULA ELLIS
VENUS FLY TRAP
SHOOT CULTURE
MS MEDIUM
KINETIN
MICROPROPAGATION