Enzymatic hydrolysis of monoterpene glycosides of passion fruit and mango with a β-glucosidase from yeast
Enzymatic hydrolysis of monoterpene glycosides of passion fruit and mango with a β-glucosidase from yeast
- Bioresource Technology, 49(3), p.243-246, 1994 .
Monoterpene glycosides extracted from passion fruit and mango were subjected to acid hydrolysis, with a phosphoric acid (H3P04)solution, and enzymatic hydrolysis using the exocellular fl-glucosidase of Candida cacaoi. The enzyme was used free and in an immobilized form covalently bonded to sodium alginate beads. Following the extraction of free and volatile compounds in the fruit juices by hydrodistillation, the bonded terpenols were hydrolyzed and the free terpenols were recovered by hydrodistillation. The fl-glucosidase enzyme hydrolysis was shown to be an effective process. Immobilized enzyme liberated less terpenols than did free enzyme.
MANGO
PASSION FRUIT
AROMA PRECURSORS
MONOTERPENYL-FL-D-GLYCOSIDES
YEAST /3-GLUCOSIDASE
ENZYMATIC RELEASE
Monoterpene glycosides extracted from passion fruit and mango were subjected to acid hydrolysis, with a phosphoric acid (H3P04)solution, and enzymatic hydrolysis using the exocellular fl-glucosidase of Candida cacaoi. The enzyme was used free and in an immobilized form covalently bonded to sodium alginate beads. Following the extraction of free and volatile compounds in the fruit juices by hydrodistillation, the bonded terpenols were hydrolyzed and the free terpenols were recovered by hydrodistillation. The fl-glucosidase enzyme hydrolysis was shown to be an effective process. Immobilized enzyme liberated less terpenols than did free enzyme.
MANGO
PASSION FRUIT
AROMA PRECURSORS
MONOTERPENYL-FL-D-GLYCOSIDES
YEAST /3-GLUCOSIDASE
ENZYMATIC RELEASE