Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae
Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae
- Plant Science, 168(1), p.45-55, 2005 .
One millimeter sized shoot-tips excised from rooted in vitro plants of the genera Musa and Ensete were successfully cryopreserved with the droplet vitrification technique.We show that the loading phase can be prolonged up to 7 h and that the optimal length of PVS2 treatment is 30-50 min at 0 8C. Ultra rapid freezing and thawing rates proved to be essential for high and reproducible post-thaw regeneration rates. This results in an increase of 23-46
BANANA
CRYOPRESERVATION
GERMPLASM COLLECTION
MERISTEM CULTURE
MUSA
VITRIFICATION
One millimeter sized shoot-tips excised from rooted in vitro plants of the genera Musa and Ensete were successfully cryopreserved with the droplet vitrification technique.We show that the loading phase can be prolonged up to 7 h and that the optimal length of PVS2 treatment is 30-50 min at 0 8C. Ultra rapid freezing and thawing rates proved to be essential for high and reproducible post-thaw regeneration rates. This results in an increase of 23-46
BANANA
CRYOPRESERVATION
GERMPLASM COLLECTION
MERISTEM CULTURE
MUSA
VITRIFICATION