Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/ evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry
Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/ evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry
- Rapid Communications in Mass Spectrometry, 22(16), p.2447-2452, 2008 .
High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MSn)was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS1-3 in positive ion mode. The MSn spectra of the [MRH]R ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-b-D-quinovopyranoside and solagenin 6-O-[b-D-xylopyranosyl-(1!3)-O-b-D-quinovopyranoside]
High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MSn)was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS1-3 in positive ion mode. The MSn spectra of the [MRH]R ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-b-D-quinovopyranoside and solagenin 6-O-[b-D-xylopyranosyl-(1!3)-O-b-D-quinovopyranoside]