A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species
A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species
- Journal of MicroBiological Methods, 61(2), p.281-284, 2005 .
A PCR-RFLP method targeted toward 26S rDNA and with 2 restriction enzymes, CfoI and BstF51, was developed to identify 11 Malassezia species. Not only type and standard strains but also 13 clinical isolates were identified successfully in this study. The results of identifications were confirmed by DNA sequencing.
MALASSEZIA
IDENTIFICATION
PCR
RFLP
CFOI
BSTF51
A PCR-RFLP method targeted toward 26S rDNA and with 2 restriction enzymes, CfoI and BstF51, was developed to identify 11 Malassezia species. Not only type and standard strains but also 13 clinical isolates were identified successfully in this study. The results of identifications were confirmed by DNA sequencing.
MALASSEZIA
IDENTIFICATION
PCR
RFLP
CFOI
BSTF51