Starch Metabolism in Shoot-forming Tobacco Callus
Starch Metabolism in Shoot-forming Tobacco Callus
- Journal of Experimental Botany, 25(85), p.288-294, 1974 .
Starch, free sugars and protein contents, and the specific activities of enzymes of starch metabolism were determined in tobacco callus cultured under shoot-forming and non-shootforming conditions. Shoot-forming cultures contained higher levels of starch, free sugars and protein. Shoot-forming cultures had higher specific activities for starch-synthesising enzymes throughout culture. On the other hand, higher levels of activity for starch-degrading enzymes in shoot-forming tissues were only observed during organized development. The role of phosphorylase in the cultured tissue was not clear.
Starch, free sugars and protein contents, and the specific activities of enzymes of starch metabolism were determined in tobacco callus cultured under shoot-forming and non-shootforming conditions. Shoot-forming cultures contained higher levels of starch, free sugars and protein. Shoot-forming cultures had higher specific activities for starch-synthesising enzymes throughout culture. On the other hand, higher levels of activity for starch-degrading enzymes in shoot-forming tissues were only observed during organized development. The role of phosphorylase in the cultured tissue was not clear.