The homeobox gene GLABRA2 affects seed oil content in Arabidopsis
The homeobox gene GLABRA2 affects seed oil content in Arabidopsis
- Plant Molecular Biology, 60, p.377-387, 2006 .
Despite a good understanding of genes involved in oil biosynthesis in seed, the mechanism(s)that controls oil accumulation is still not known. To identify genes that control oil accumulation in seed, we have developed a simple screening method to isolate Arabidopsis seed oil mutants. The method includes an initial screen for seed density followed by a seed oil screen using an automated Nuclear Magnetic Resonance (NMR). Using this method, we isolated ten low oil mutants and one high oil mutant. The high oil mutant, p777, accumulated 8
DENSITY SCREEN
GLABRA 2
HIGH OIL
MUCILAGE
TRICHOME
Despite a good understanding of genes involved in oil biosynthesis in seed, the mechanism(s)that controls oil accumulation is still not known. To identify genes that control oil accumulation in seed, we have developed a simple screening method to isolate Arabidopsis seed oil mutants. The method includes an initial screen for seed density followed by a seed oil screen using an automated Nuclear Magnetic Resonance (NMR). Using this method, we isolated ten low oil mutants and one high oil mutant. The high oil mutant, p777, accumulated 8
DENSITY SCREEN
GLABRA 2
HIGH OIL
MUCILAGE
TRICHOME