Identification of Alternaria brassicicola genes expressed in planta during pathogenesis of Arabidopsis thaliana
Identification of Alternaria brassicicola genes expressed in planta during pathogenesis of Arabidopsis thaliana
- Fungal Genetics and Biology, 41(2), p.115-128, 2004 .
Alternaria brassicicola is a necrotrophic fungal pathogen that causes black spot disease on cruciferous plants including economically important Brassica species. The purpose of this study was to identify fungal genes expressed during infection of Arabidopsis. In order to identify candidate genes involved in pathogenicity, we employed suppression subtractive hybridization (SSH)between RNA isolated from A. brassicicola spores incubated in water and on the leaf surface of the Arabidopsis ecotype Landsberg. Two populations of cDNA were created from total RNA extracted after 24 h when approximately 80
ALTERNARIA BRASSICICOLA
ARABIDOPSIS THALIANA
BRASSICA OLERACEAE
PATHOGENICITY
SUPPRESSION SUBTRACTIVE HYBRIDIZATION
Alternaria brassicicola is a necrotrophic fungal pathogen that causes black spot disease on cruciferous plants including economically important Brassica species. The purpose of this study was to identify fungal genes expressed during infection of Arabidopsis. In order to identify candidate genes involved in pathogenicity, we employed suppression subtractive hybridization (SSH)between RNA isolated from A. brassicicola spores incubated in water and on the leaf surface of the Arabidopsis ecotype Landsberg. Two populations of cDNA were created from total RNA extracted after 24 h when approximately 80
ALTERNARIA BRASSICICOLA
ARABIDOPSIS THALIANA
BRASSICA OLERACEAE
PATHOGENICITY
SUPPRESSION SUBTRACTIVE HYBRIDIZATION