Development of embryogenic cell suspensions from shoot meristematic tissue in bananas and plantains (Musa spp.)
Development of embryogenic cell suspensions from shoot meristematic tissue in bananas and plantains (Musa spp.)
- Plant Science, 170, p.104-112, 2006 .
Multiple meristem cultures of 18 varieties belonging to 5 genome types in Musa (AA, AAA, AAA-h, AAB, ABB)were established by culturing elongated shoots on MS medium supplemented with 100 mM BAP. The top layers comprising the most meristematic tissue, i.e. scalps, were excised and induced for embryogenesis on media containing 1-50 mM 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic responses were obtained for each of the tested concentrations, with an optimum at 5 mM 2,4-D. From the 24,375 scalps tested, only 3.3
EMBRYOGENIC FREQUENCY
EMBRYOGENIC CELL SUSPENSIONS
MERISTEMATIC TISSUE (SCALP)
MUSA
SOMATIC EMBRYOGENESIS
PLANT REGENERATION
Multiple meristem cultures of 18 varieties belonging to 5 genome types in Musa (AA, AAA, AAA-h, AAB, ABB)were established by culturing elongated shoots on MS medium supplemented with 100 mM BAP. The top layers comprising the most meristematic tissue, i.e. scalps, were excised and induced for embryogenesis on media containing 1-50 mM 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic responses were obtained for each of the tested concentrations, with an optimum at 5 mM 2,4-D. From the 24,375 scalps tested, only 3.3
EMBRYOGENIC FREQUENCY
EMBRYOGENIC CELL SUSPENSIONS
MERISTEMATIC TISSUE (SCALP)
MUSA
SOMATIC EMBRYOGENESIS
PLANT REGENERATION