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Separation efficiencies in hydrophilic interaction chromatography - Journal of Chromatography A , 184(1-2), p.474-503, 2008 .

Hydrophilic interaction chromatography (HILIC)is important for the separation of highly polar substances including biologically active compounds, such as pharmaceutical drugs, neurotransmitters, nucleosides, nucleotides, amino acids, peptides, proteins, oligosaccharides, carbohydrates, etc. In the HILIC mode separation, aqueous organic solvents are used as mobile phases on more polar stationary phases that consist of bare silica, and silica phases modified with amino, amide, zwitterionic functional group, polyols including saccharides and other polar groups. This review discusses the column efficiency of HILIC materials in relation to solute and stationary phase structures, as well as comparisons between particlepacked and monolithic columns. In addition, a literature review consisting of 2006-2007 data is included, as a follow up to the excellent review by Hemstr¨om and Irgum

Subjects--Topical Terms:
HILIC
SEPARATION BEFFICIENCY
BARE SILICA
AMINO-SILICA
AMIDE-SILICA
POLY(SUCCINIMIDE)-SILICA
SULFOALKYLBETAINE-SILICA
DIOL-SILICA
CD-SILICA
POLYMER MONOLITHIC COLUMNS
SILICA MONOLITHIC COLUMN