Chemical attachment of porcine pancreatic lipase to crosslinked poly(vinyl alcohol)by means of adipoyldichloride
Chemical attachment of porcine pancreatic lipase to crosslinked poly(vinyl alcohol)by means of adipoyldichloride
- Process BioChemistry, 38(5), p.641-647, 2002 .
The biotechnological applications of lipases as biocatalysts for esterification, interesterification and ester hydrolysis reaction requires simple but efficient methods to immobilize the enzyme, yielding highly stable and active biocatalysts which are easy to recover. In this study we present a new method to achieve lipase immobilization by covalent attachment to crosslinked poly(vinyl alcohol)(CL-PVA). Porcine pancreatic lipase was immobilized on CL-PVA by crosslinking with adipoyldichloride with the activity yield of 63
POLY(VINYL ALCOHOL)
PORCINE PANCREATIC LIPASE
IMMOBILIZATION
CROSS-LINKING
COVALENT ATTACHMENT
The biotechnological applications of lipases as biocatalysts for esterification, interesterification and ester hydrolysis reaction requires simple but efficient methods to immobilize the enzyme, yielding highly stable and active biocatalysts which are easy to recover. In this study we present a new method to achieve lipase immobilization by covalent attachment to crosslinked poly(vinyl alcohol)(CL-PVA). Porcine pancreatic lipase was immobilized on CL-PVA by crosslinking with adipoyldichloride with the activity yield of 63
POLY(VINYL ALCOHOL)
PORCINE PANCREATIC LIPASE
IMMOBILIZATION
CROSS-LINKING
COVALENT ATTACHMENT