Rapid sample preparation technique for the determination of pyrrolizidine alkaloids in plant extracts
Rapid sample preparation technique for the determination of pyrrolizidine alkaloids in plant extracts
- Journal of Chromatography A, 668(2), p.427-433, 1994 .
To determine toxic pyrrolizidine alkaloids from various plant sources, a sample preparation technique is described that affords alkaloid fractions suitable for capillary GC or TLC determination. The procedure includes the reduction of the alkaloid N-oxides with the oxygen-absorbing resin Serdoxit and a clean-up with strong cation-exchange solid-phase columns. If desired an enrichment of the alkaloids can be obtained. The method was tested with methanolic extracts from Senecio vulgaris, Petasites hybridus and Symphytum ofjcinale.
To determine toxic pyrrolizidine alkaloids from various plant sources, a sample preparation technique is described that affords alkaloid fractions suitable for capillary GC or TLC determination. The procedure includes the reduction of the alkaloid N-oxides with the oxygen-absorbing resin Serdoxit and a clean-up with strong cation-exchange solid-phase columns. If desired an enrichment of the alkaloids can be obtained. The method was tested with methanolic extracts from Senecio vulgaris, Petasites hybridus and Symphytum ofjcinale.