Sequence-independent amplification and cloning of large dsRNA virus genome segments by poly(dA)-oligonucleotide ligation
Sequence-independent amplification and cloning of large dsRNA virus genome segments by poly(dA)-oligonucleotide ligation
- Journal of Virological Methods, 72(2), p.243-247, 1998 .
A strategy was developed for sequence-independent synthesis and amplification of full-length cDNA of 3-4 kb genes of dsRNA viruses. The method of single primer amplification (Lambden et al., 1992)was adapted by the inclusion of a 3
DSRNA VIRUSES
AFRICAN HORSE SICKNESS VIRUS (AHSV)
SINGLE PRIMER AMPLIFICATION
A strategy was developed for sequence-independent synthesis and amplification of full-length cDNA of 3-4 kb genes of dsRNA viruses. The method of single primer amplification (Lambden et al., 1992)was adapted by the inclusion of a 3
DSRNA VIRUSES
AFRICAN HORSE SICKNESS VIRUS (AHSV)
SINGLE PRIMER AMPLIFICATION