Proteinase and exopeptidase hydrolysis of whey protein: Comparison of the TNBS, OPA and pH stat methods for quantification of degree of hydrolysis
Proteinase and exopeptidase hydrolysis of whey protein: Comparison of the TNBS, OPA and pH stat methods for quantification of degree of hydrolysis
- International Dairy Journal, 13(6), p.447-453, 2003 .
Whey protein hydrolysates were generated with Alcalase 2.4L and Debitrase HYW20 which are proteinase and exopeptidase enriched enzyme preparations, respectively. Degree of hydrolysis (DH)values were quantified with the TNBS, OPA and pH stat methods. Poor correlation was observed between the three methods for DH values in Debitrase HYW20 hydrolysates. For Alcalase 2.4L hydrolysates, the OPA method gave DH values that were approximately 15
WHEY PROTEIN HYDROLYSATES
DEGREE OF HYDROLYSIS
PROTEINASE
EXOPEPTIDASE
Whey protein hydrolysates were generated with Alcalase 2.4L and Debitrase HYW20 which are proteinase and exopeptidase enriched enzyme preparations, respectively. Degree of hydrolysis (DH)values were quantified with the TNBS, OPA and pH stat methods. Poor correlation was observed between the three methods for DH values in Debitrase HYW20 hydrolysates. For Alcalase 2.4L hydrolysates, the OPA method gave DH values that were approximately 15
WHEY PROTEIN HYDROLYSATES
DEGREE OF HYDROLYSIS
PROTEINASE
EXOPEPTIDASE