In vitro DNA methylation by wheat nuclear cytosine DNA methyltransferase: effect of phytohormones
In vitro DNA methylation by wheat nuclear cytosine DNA methyltransferase: effect of phytohormones
- Gene, 157(1-2), p.279-281, 1955 .
Cytosine DNA methyltransferases (MTases)were isolated from nuclei of wheat seedlings and germinating embryos. The MTases isolated from both sources were able to perform de novo and maintenance DNA methylations. The most purified MTase fraction showed the presence of one main 67-kDa protein (embryos)and of a 85-kDa protein (in seedlings)in SDS-PAGE. Some plant growth regulators (gibberellic acid A3, 6-benzylaminopurine nd fusicoccin)elevate by 30-65
Cytosine DNA methyltransferases (MTases)were isolated from nuclei of wheat seedlings and germinating embryos. The MTases isolated from both sources were able to perform de novo and maintenance DNA methylations. The most purified MTase fraction showed the presence of one main 67-kDa protein (embryos)and of a 85-kDa protein (in seedlings)in SDS-PAGE. Some plant growth regulators (gibberellic acid A3, 6-benzylaminopurine nd fusicoccin)elevate by 30-65