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Selective Isolation of Mercurated DNA by Affinity Chromatography on Thiol Matrices'

Selective Isolation of Mercurated DNA by Affinity Chromatography on Thiol Matrices' - Analytical BioChemistry, 146(1), p.64-70, 1985 .

A method for isolating picomole quantities of nascent mercurated DNA from a mixture of cellular nucleic acids using affinity chromatography on thiol-agarose is described. Analysis of mercurated DNA (HgDNA)isolated in the presence of in v-labeled cellular RNA or in vitrosynthesized RNA showed a low level of RNA contamination, about 0.04-0.16

Subjects--Topical Terms:
AFFINITY CHROMATOGRAPHY
AFFINITY LABELING
DNA POLYMERASE
DNA
PURINES AND PYRIMIDINES
NUCLEIC ACID CHEMISTRY

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