Separation of glutaraldehyde and some of its aldol condensation products by hydroxyl-aldehyde group affinity chromatography
Separation of glutaraldehyde and some of its aldol condensation products by hydroxyl-aldehyde group affinity chromatography
- Journal of BioChemical and Biophysical Methods, 22(4), p.321-329, 1991 .
Glutaraldeh)dc exists in aqueous solution in equilibrium with monomers and polymers of cyclic and open-chain hemialdals and hydrates. At alkaline pH oligomeric and polymeric a,fl-unsaturated aldehyde derivatives are formed from primarily produced aldol condensation products. This communication reports a method for separation of such aldol condensates by means of a new high performance liquid chromatography technique based on the affinity of aldehyde groups for hydroxyl groups of a hydroxylated polyether matrix (Bio-Gel SEC-10). Five peaks corresponding to different aldol condensates of glutaraldehyde were obtained from the affinity column. They have been distinguished by ~tt-NMR and UV spectroscopy. Kinetic measurements yielded formation rates for the different aldol condensates.
GLUTARALDEHYDE
CONDENSATION PRODUCT
OLIGOMERIC
POLYMERIC
SEPARATION
PARTIAL CHARACTERIZATION
HYDROXYL-ALDEHYDE GROUP
INTERACTION CHROMATOGRAPHY
Glutaraldeh)dc exists in aqueous solution in equilibrium with monomers and polymers of cyclic and open-chain hemialdals and hydrates. At alkaline pH oligomeric and polymeric a,fl-unsaturated aldehyde derivatives are formed from primarily produced aldol condensation products. This communication reports a method for separation of such aldol condensates by means of a new high performance liquid chromatography technique based on the affinity of aldehyde groups for hydroxyl groups of a hydroxylated polyether matrix (Bio-Gel SEC-10). Five peaks corresponding to different aldol condensates of glutaraldehyde were obtained from the affinity column. They have been distinguished by ~tt-NMR and UV spectroscopy. Kinetic measurements yielded formation rates for the different aldol condensates.
GLUTARALDEHYDE
CONDENSATION PRODUCT
OLIGOMERIC
POLYMERIC
SEPARATION
PARTIAL CHARACTERIZATION
HYDROXYL-ALDEHYDE GROUP
INTERACTION CHROMATOGRAPHY