Comparison of Plasma Membrane Proteomic Changes of Arabidopsis Suspension-Cultured Cells (T87 Line)after Cold and ABA Treatment in Association with Freezing Tolerance Development
Comparison of Plasma Membrane Proteomic Changes of Arabidopsis Suspension-Cultured Cells (T87 Line)after Cold and ABA Treatment in Association with Freezing Tolerance Development
- Plant & Cell Physiology, 53(3), p.543-554, 2012 .
The plasma membrane (PM)is the primary site of freezing injury in plants. To determine global changes in PM protein profiles in association with freezing tolerance development, proteome analysis of the purified PM of Arabidopsis suspension-cultured cells (T87 line)was conducted with label-free protein quantification technology. Freezing tolerance of Arabidopsis cells at the lag growth phase (8 d old)increased after cold acclimation (CA)or ABA treatment. Proteome analysis assigned 658 proteins in the PM in total, of which 45.3
ABA
COLD ACCLIMATION
LABEL-FREE PROTEIN QUANTIFICATION
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY/MASS SPECTROMETRY
PLASMA MEMBRANE
PROTEOMICS
The plasma membrane (PM)is the primary site of freezing injury in plants. To determine global changes in PM protein profiles in association with freezing tolerance development, proteome analysis of the purified PM of Arabidopsis suspension-cultured cells (T87 line)was conducted with label-free protein quantification technology. Freezing tolerance of Arabidopsis cells at the lag growth phase (8 d old)increased after cold acclimation (CA)or ABA treatment. Proteome analysis assigned 658 proteins in the PM in total, of which 45.3
ABA
COLD ACCLIMATION
LABEL-FREE PROTEIN QUANTIFICATION
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY/MASS SPECTROMETRY
PLASMA MEMBRANE
PROTEOMICS