Improving Culture Conditions for Temporomandibular Joint Disc Tissue Engineering
Improving Culture Conditions for Temporomandibular Joint Disc Tissue Engineering
- Cells Tissues Organs, 185(4), p.246-257, 2007 .
Background: The temporomandibular joint (TMJ)is ex-tremely important for activities like eating and talking, which can become painful and difficult for patients with TMJ dys-function. Tissue engineering is a potential alternative to cur-rent surgical interventions through replacement of diseased or injured tissue with a functional construct. Since research with TMJ disc cells began relatively recently, optimal cultur-ing conditions must be determined. Methods: Metabolic additives, L -glutamine, L -alanyl-L -glutamine, sodium pyru-vate, and insulin, were examined for their effects on TMJ disc cells in monolayer. Effects of L -proline were examined in three-dimensional (3-D)culture at concentrations of 0, 25 and 100 mg/l. Results: The combination of L -glutamine, so-dium pyruvate, and insulin improved cell proliferation rates without affecting collagen production or gene expression. No differences were observed in mechanical properties of the engineered constructs; however, collagen and glycos-aminoglycan quantities normalized to cell number de-creased at the highest concentration of L -proline. Conclu-sion: This work identified supplements for 2-D monolayer expansion. Other supplements or culture conditions still need to be investigated for 3-D tissue production. This work improves upon porcine TMJ disc cell culturing conditions, taking us closer to being able to engineer the TMJ disc.
TEMPOROMANDIBULAR JOINT DISC
TISSUE ENGINEERING
TISSUE CULTURE
Background: The temporomandibular joint (TMJ)is ex-tremely important for activities like eating and talking, which can become painful and difficult for patients with TMJ dys-function. Tissue engineering is a potential alternative to cur-rent surgical interventions through replacement of diseased or injured tissue with a functional construct. Since research with TMJ disc cells began relatively recently, optimal cultur-ing conditions must be determined. Methods: Metabolic additives, L -glutamine, L -alanyl-L -glutamine, sodium pyru-vate, and insulin, were examined for their effects on TMJ disc cells in monolayer. Effects of L -proline were examined in three-dimensional (3-D)culture at concentrations of 0, 25 and 100 mg/l. Results: The combination of L -glutamine, so-dium pyruvate, and insulin improved cell proliferation rates without affecting collagen production or gene expression. No differences were observed in mechanical properties of the engineered constructs; however, collagen and glycos-aminoglycan quantities normalized to cell number de-creased at the highest concentration of L -proline. Conclu-sion: This work identified supplements for 2-D monolayer expansion. Other supplements or culture conditions still need to be investigated for 3-D tissue production. This work improves upon porcine TMJ disc cell culturing conditions, taking us closer to being able to engineer the TMJ disc.
TEMPOROMANDIBULAR JOINT DISC
TISSUE ENGINEERING
TISSUE CULTURE