DNA Sequencing: An Outsourcing Guide
DNA Sequencing: An Outsourcing Guide
- Current Protocols Essential Laboratory Techniques, 2(1), p.12-1, 2009 .
DNA sequencing, the process of determining the precise order of nucleotides in a DNA strand, is a fundamental and ubiquitous method in molecular biology. Examples of its manyusesincludecharacterizationofunknownDNA,mutationdetection,alleleidentifi-cation,andcloneconstructconfirmation.Advancesinsequencingtechnology,motivated by the successful International Human Genome Project, have resulted in unprecedented access to high-quality and cost-effective automated analysis. It is increasingly common for researchers to delegate routine DNA sequencing needs to dedicated laboratories or companiesspecializinginthistechnology.Inthisunit,wediscusskeyprotocolsformax-imizing the success of DNA sequencing when working with such service providers and describe detailed troubleshooting advice for analyzing anomalous results.
DNA sequencing, the process of determining the precise order of nucleotides in a DNA strand, is a fundamental and ubiquitous method in molecular biology. Examples of its manyusesincludecharacterizationofunknownDNA,mutationdetection,alleleidentifi-cation,andcloneconstructconfirmation.Advancesinsequencingtechnology,motivated by the successful International Human Genome Project, have resulted in unprecedented access to high-quality and cost-effective automated analysis. It is increasingly common for researchers to delegate routine DNA sequencing needs to dedicated laboratories or companiesspecializinginthistechnology.Inthisunit,wediscusskeyprotocolsformax-imizing the success of DNA sequencing when working with such service providers and describe detailed troubleshooting advice for analyzing anomalous results.