Cultivation and Preservation of Methanogenic Bacteria
Cultivation and Preservation of Methanogenic Bacteria
- Zentralblatt Mikrobiologie , 143(2), p.149-155, 1988 .
Medium requirements and growth yields of Methanosarcina barkeri, Methanospirillum hungatei, Methanococcus vanielii and Methanobacterium /ormicicum were evaluated. All strains grew well in completely defined media; only M. barkeri required additional vitamins. The maximum achievable biomass yields with H2/C02 (80: 20)as an energy source were 1.5 g dry weight/I. Gas transfer is the limiting factor for growth as was determined in the case of the fermentation of M. vannielii. All strains can be preserved in a viable state either at -20°C or in liquid nitrogen provided that cells from the exponential phase of growth are used. The addition of 2 g cysteine/l to the media results in floculation of M. bryantii and M. /ormicicum.
Medium requirements and growth yields of Methanosarcina barkeri, Methanospirillum hungatei, Methanococcus vanielii and Methanobacterium /ormicicum were evaluated. All strains grew well in completely defined media; only M. barkeri required additional vitamins. The maximum achievable biomass yields with H2/C02 (80: 20)as an energy source were 1.5 g dry weight/I. Gas transfer is the limiting factor for growth as was determined in the case of the fermentation of M. vannielii. All strains can be preserved in a viable state either at -20°C or in liquid nitrogen provided that cells from the exponential phase of growth are used. The addition of 2 g cysteine/l to the media results in floculation of M. bryantii and M. /ormicicum.