In vitro induction of triploid roots and shoots from mature endosperm of Jatropha pandureafolia
In vitro induction of triploid roots and shoots from mature endosperm of Jatropha pandureafolia
- Zeitschrift für Pflanzenphysiologie, 66, p.93-96, 1971 .
The mature endosperm of J. panduraefolia proliferated profusely on modified White's medium + 2,4-D + kinetin + YE. The tissue thus obtained grew compact upon continuous subculture and differentiated vascular cells. When callus pieces were transferred to WB + NAA + kinetin + CH, shoot buds and roots differentiated all round the callus. On WB + CH or WB + an auxin, only roots differentiated and on WB + kinetin, only shoots differentiated. In some cultures roots and shoots differentiated from the same callus. Anatomy of the in vitro-differentiated roots and shoots was comparable to in vivo Structures. Cytological preparations of Il-month-old callus showed polyploid cells and chromosomal abnormalities. My results indicate that mature endosperm can also differentiate into triploid organs.
The mature endosperm of J. panduraefolia proliferated profusely on modified White's medium + 2,4-D + kinetin + YE. The tissue thus obtained grew compact upon continuous subculture and differentiated vascular cells. When callus pieces were transferred to WB + NAA + kinetin + CH, shoot buds and roots differentiated all round the callus. On WB + CH or WB + an auxin, only roots differentiated and on WB + kinetin, only shoots differentiated. In some cultures roots and shoots differentiated from the same callus. Anatomy of the in vitro-differentiated roots and shoots was comparable to in vivo Structures. Cytological preparations of Il-month-old callus showed polyploid cells and chromosomal abnormalities. My results indicate that mature endosperm can also differentiate into triploid organs.