An automated quantitative assay for fungal growth inhibition
An automated quantitative assay for fungal growth inhibition
- FEMS MicroBiology Letter, 69(1-2), p.55-59, 1990 .
A simple technique which enables the monitor ing of fungal growth with the aid of a microplate reader is described. In the absorbance range of 0to 0,6 units, a straight-line relationship exists between absorbance at 595 nm and dry weight of micro#ate cultures, indicating that culture absorbance is an accurate indicator of fungal biomass. The relative standard deviatioq of the absorbanc¢ measurements was low (typically between 2 and 6 percent)when spores were used for in0culum. With inoculi consisting of mycelial fragments, slightly higher standard deviations (ca. 10 percent)were found. The microplate reader technique is particularly suited for determination of growth inhibition curves, since it is extremely fast. reliable, and requites as little as 75 ~ul of total culture volume.
GROWTH INHIBITION ASSAYS
FILAMENTOUS FUNGI
MICROPLATE READER
A simple technique which enables the monitor ing of fungal growth with the aid of a microplate reader is described. In the absorbance range of 0to 0,6 units, a straight-line relationship exists between absorbance at 595 nm and dry weight of micro#ate cultures, indicating that culture absorbance is an accurate indicator of fungal biomass. The relative standard deviatioq of the absorbanc¢ measurements was low (typically between 2 and 6 percent)when spores were used for in0culum. With inoculi consisting of mycelial fragments, slightly higher standard deviations (ca. 10 percent)were found. The microplate reader technique is particularly suited for determination of growth inhibition curves, since it is extremely fast. reliable, and requites as little as 75 ~ul of total culture volume.
GROWTH INHIBITION ASSAYS
FILAMENTOUS FUNGI
MICROPLATE READER