Somatic Embryogenesis in Tissues From Mesophyll and Cell Suspension Protoplasts of Medicago coerulea and M. glutinosa
Somatic Embryogenesis in Tissues From Mesophyll and Cell Suspension Protoplasts of Medicago coerulea and M. glutinosa
- Z. Pjlanzenphysiol., 106, p.105-110, 1982 .
Mesophyll and cell suspension protoplasts of Medicago coerulea and M glutinosa divided to form callus. The conditions of plant growth, plant age, protoplast isolation, and protoplast cul-ture were critical for sustained division of mesophyll protoplasts. Those for division of cell suspension protoplasts were less stringent. 2,4-D in combination with a cytokinin induced embryogenesis in both Medicago species. In M. coerulea, BAP was effective as the cytokinin, while M. glutinosa had a requirement for zeatin. The potential of protoplast systems for transfer of characteristics from M. coerulea to M. glutinosa and M. sativa is discussed.
MEDICAGO COERULEA
M GLUTINOSA
BAP
CELL SUSPENSION PROTOPLASTS
MESOPHYLL PROTOPLASTS
DIVISION
2,4·D
FORAGE LEGUMES
GENETIC MANIPULATION
SOMATIC EMBRYOGENESIS
ZEATIN
Mesophyll and cell suspension protoplasts of Medicago coerulea and M glutinosa divided to form callus. The conditions of plant growth, plant age, protoplast isolation, and protoplast cul-ture were critical for sustained division of mesophyll protoplasts. Those for division of cell suspension protoplasts were less stringent. 2,4-D in combination with a cytokinin induced embryogenesis in both Medicago species. In M. coerulea, BAP was effective as the cytokinin, while M. glutinosa had a requirement for zeatin. The potential of protoplast systems for transfer of characteristics from M. coerulea to M. glutinosa and M. sativa is discussed.
MEDICAGO COERULEA
M GLUTINOSA
BAP
CELL SUSPENSION PROTOPLASTS
MESOPHYLL PROTOPLASTS
DIVISION
2,4·D
FORAGE LEGUMES
GENETIC MANIPULATION
SOMATIC EMBRYOGENESIS
ZEATIN