A rapid and efficient transformation method for the production of large numbers of transgenic potato plants
A rapid and efficient transformation method for the production of large numbers of transgenic potato plants
- Plant Science, 63(1), p.79-85, 1989 .
A procedure is described for the rapid and efficient production of transgenic potato (Solanum tuberosum L.)plants using Agrobacterium-based vectors. The key factors are the genotype and the level of kanamycin sulfate used for selection. Optimized culture conditions easily and routinely permitted the isolation of hundreds of individual regenerates expressing ß-glucuronidase (GUS)transcriptionally fused to promoters from cauliflower mosaic virus (CaMV 35S)or a class-I patatin gene.
CAULIFLOWER MOSAIC VIRUS 35S PROMOTER
PATATIN PROMOTER
POTATO
TRANSGENIC PLANTS
B-GLUCURONIDASE
A procedure is described for the rapid and efficient production of transgenic potato (Solanum tuberosum L.)plants using Agrobacterium-based vectors. The key factors are the genotype and the level of kanamycin sulfate used for selection. Optimized culture conditions easily and routinely permitted the isolation of hundreds of individual regenerates expressing ß-glucuronidase (GUS)transcriptionally fused to promoters from cauliflower mosaic virus (CaMV 35S)or a class-I patatin gene.
CAULIFLOWER MOSAIC VIRUS 35S PROMOTER
PATATIN PROMOTER
POTATO
TRANSGENIC PLANTS
B-GLUCURONIDASE