Chapter 3 Use of Bioinformatics in Planning a Protein Purification
Chapter 3 Use of Bioinformatics in Planning a Protein Purification
- Methods in Enzymology, 463(C), p.21-28, 2009 .
Now that many hundreds and even thousands of whole genomes have been sequenced, it is rare to be studying a target protein whose amino acid sequence is not known. However, it is still often necessary to obtain large amounts of the target protein for a variety of purposes including structural studies, drug discovery, enzymology, protein biochemistry, and industrial application. It would seem that knowing the amino acid sequences would make it much easier to design an effective purification of that protein. We examine in this chapter what you can and cannot predict from an amino acid sequence and conclude that protein purification is still largely an empirical science. © 2009 Elsevier Inc. All rights reserved
AMINO ACID SEQUENCE
BIOINFORMATICS
ENZYMOLOGY
GENE SEQUENCE
GENOME
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN PURIFICATION
PROTEIN STRUCTURE
REVIEW
ALGORITHM
ANIMAL
BIOLOGY
CHEMISTRY
HUMAN
HUMAN
METABOLISM
METHODOLOGY
PHYSIOLOGY
PROTEIN CONFORMATION
PROTEIN DENATURATION
PROTEIN MULTIMERIZATION
PROTEIN PROCESSING
Now that many hundreds and even thousands of whole genomes have been sequenced, it is rare to be studying a target protein whose amino acid sequence is not known. However, it is still often necessary to obtain large amounts of the target protein for a variety of purposes including structural studies, drug discovery, enzymology, protein biochemistry, and industrial application. It would seem that knowing the amino acid sequences would make it much easier to design an effective purification of that protein. We examine in this chapter what you can and cannot predict from an amino acid sequence and conclude that protein purification is still largely an empirical science. © 2009 Elsevier Inc. All rights reserved
AMINO ACID SEQUENCE
BIOINFORMATICS
ENZYMOLOGY
GENE SEQUENCE
GENOME
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN PURIFICATION
PROTEIN STRUCTURE
REVIEW
ALGORITHM
ANIMAL
BIOLOGY
CHEMISTRY
HUMAN
HUMAN
METABOLISM
METHODOLOGY
PHYSIOLOGY
PROTEIN CONFORMATION
PROTEIN DENATURATION
PROTEIN MULTIMERIZATION
PROTEIN PROCESSING