Chapter 4 Preparing a Purification Summary Table
Chapter 4 Preparing a Purification Summary Table
- Methods in Enzymology, 463(C), p.29-34, 2009 .
Once a protein purification scheme has been developed, the purification, characterization, and use/structure of a target protein are usually published. It is highly desirable to present the major steps in the purification and the corresponding features of the protein at each step summarized in the form of a purification summary table. In considering whether to repeat a published protein purification, a reader needs this information to evaluate the purification, and to decide if it is worth following or if it needs major modifications. In this chapter, I discuss the main characteristics of a useful purification summary table and point out common mistakes and problems I see in many such tables. © 2009 Elsevier Inc. All rights reserved.
NONHUMAN
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN PURIFICATION
PROTEIN STRUCTURE
REVIEW
ANIMAL
CELL FRACTIONATION
CHEMISTRY
HUMAN
ISOLATION AND PURIFICATION
METHODOLOGY
POLYACRYLAMIDE GEL ELECTROPHORESIS
STATISTICS
Once a protein purification scheme has been developed, the purification, characterization, and use/structure of a target protein are usually published. It is highly desirable to present the major steps in the purification and the corresponding features of the protein at each step summarized in the form of a purification summary table. In considering whether to repeat a published protein purification, a reader needs this information to evaluate the purification, and to decide if it is worth following or if it needs major modifications. In this chapter, I discuss the main characteristics of a useful purification summary table and point out common mistakes and problems I see in many such tables. © 2009 Elsevier Inc. All rights reserved.
NONHUMAN
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN PURIFICATION
PROTEIN STRUCTURE
REVIEW
ANIMAL
CELL FRACTIONATION
CHEMISTRY
HUMAN
ISOLATION AND PURIFICATION
METHODOLOGY
POLYACRYLAMIDE GEL ELECTROPHORESIS
STATISTICS