Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
- Analytical BioChemistry, 162(1), p.156-159, 1987 .
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
GENE EXPRESSION
HUMAN CELLS
MESSENGER RNA
NUCLEIC ACIDS
PURIFICATION
RNA
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
GENE EXPRESSION
HUMAN CELLS
MESSENGER RNA
NUCLEIC ACIDS
PURIFICATION
RNA