Efficacy of RNA-spray-induced silencing of Phakopsora pachyrhizi chitin synthase genes to control soybean rust
Efficacy of RNA-spray-induced silencing of Phakopsora pachyrhizi chitin synthase genes to control soybean rust
- Journal of General Plant Pathology, 88(3), p.203-206, 2022 .
Asian soybean rust caused by Phakopsora pachyrhizi is mainly controlled by synthetic fungicides; however, fungicide tolerance has emerged in P. pachyrhizi. In tests of spray-induced gene silencing (SIGS)using dsRNAs of P. pachyrhizi chitin synthase genes (dsCHS), which are important for the differentiation of appressoria that are needed for infection, soybean rust lesion formation was reduced. Further, treatment with dsCHS suppressed appressoria formation. These results suggest that SIGS of fungal CHS genes will provide an additional tool to control soybean rust.
ASIAN SOYBEAN RUST
CHITIN SYNTHASE
DOUBLE-STRANDED RNA
PHAKOPSORA PACHYRHIZI
SOYBEAN
SPRAYINDUCED-GENE SILENCING
Asian soybean rust caused by Phakopsora pachyrhizi is mainly controlled by synthetic fungicides; however, fungicide tolerance has emerged in P. pachyrhizi. In tests of spray-induced gene silencing (SIGS)using dsRNAs of P. pachyrhizi chitin synthase genes (dsCHS), which are important for the differentiation of appressoria that are needed for infection, soybean rust lesion formation was reduced. Further, treatment with dsCHS suppressed appressoria formation. These results suggest that SIGS of fungal CHS genes will provide an additional tool to control soybean rust.
ASIAN SOYBEAN RUST
CHITIN SYNTHASE
DOUBLE-STRANDED RNA
PHAKOPSORA PACHYRHIZI
SOYBEAN
SPRAYINDUCED-GENE SILENCING