DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format
DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format
- Cold Spring Harbor Protocols, 2010(11), p.pdb-prot5516, 2010 .
This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB)and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR)procedures.
This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB)and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR)procedures.