Acridonepoxidgehalte in Kalluskulturen von Ruta graveolens und ihre Steigerung durch Mischkultur mit Pilzen
Acridonepoxidgehalte in Kalluskulturen von Ruta graveolens und ihre Steigerung durch Mischkultur mit Pilzen
- Z Naturforsch C, 37, p.575-583, 1982 .
The classic attempt to find optimum culture conditions lead to hormonautotrophic cultures and to dark grown cultures. We made the new attempt to rise the yield of rutacridone-epoxides by coculture with not hostspecific fungi. Conditions were chosen that only diffusible elicitors could influence callus tissue. 14 days of coculture mostly caused reduced acridone-epoxide content, growthrate and up to total disappearance of chlorophyll. Within 3 - 7 days of coculture some fungi induced a significant rise of acridone-epoxide content (up to 50-fold). Culture filtrates of these fungi showed comparable effects. Our results proved the acridone-epoxides to be phytoalexins. The use of fungal elicitors could be a new method to increase secondary product accumulation in tissue culture.
ACRIDONE-EPOXIDE
CELL CULTURE
COCULTURE WITH FUNGI
PHYTOALEXIN
RUTA GRAVEOLENS
The classic attempt to find optimum culture conditions lead to hormonautotrophic cultures and to dark grown cultures. We made the new attempt to rise the yield of rutacridone-epoxides by coculture with not hostspecific fungi. Conditions were chosen that only diffusible elicitors could influence callus tissue. 14 days of coculture mostly caused reduced acridone-epoxide content, growthrate and up to total disappearance of chlorophyll. Within 3 - 7 days of coculture some fungi induced a significant rise of acridone-epoxide content (up to 50-fold). Culture filtrates of these fungi showed comparable effects. Our results proved the acridone-epoxides to be phytoalexins. The use of fungal elicitors could be a new method to increase secondary product accumulation in tissue culture.
ACRIDONE-EPOXIDE
CELL CULTURE
COCULTURE WITH FUNGI
PHYTOALEXIN
RUTA GRAVEOLENS