CRISPR-Cas13d in plant biology: an insight.
CRISPR-Cas13d in plant biology: an insight.
- Plant Biotechnology Reports. 18(3), 301-311, 2024, DOI: https://doi.org/10.1007/s11816-024-00893-6 .
Plants are among the many creatures that have benefited from the widespread application of the CRISPR-associated Cas system as a genome-editing tool for investigating gene function, identifying disease, and enhancing agricultural yields. Although the CRISPR/Cas systems for DNA editing are widely employed, post-transcriptional manipulation of RNA remains difficult despite the prevalence of Cas9. Type VI CRISPR/Cas systems, which were recently found, allow for precise RNA editing without permanently affecting the genome. Cas13d has been put to good use in RNA-related studies across a wide range of RNA knock-down, and RNA detection without affecting DNA. Regulation of cas13d specificity and activity helps to avoid the off-target effects and immune responses in plants. Cas13d as highly efficient RNA-targeting tools for the virus resistance, gene function studies, disease diagnostics, and crop improvement in plants. However, CRISPR/Cas13d applications in plant RNA biology are just getting started. This article discusses how RNA editing tools derived from the CRISPR/Cas13d system are currently being used and where they may be used in the future for plant research.
CRISPR
CAS13D
PLANT BIOLOGY
RNA EDITING
Plants are among the many creatures that have benefited from the widespread application of the CRISPR-associated Cas system as a genome-editing tool for investigating gene function, identifying disease, and enhancing agricultural yields. Although the CRISPR/Cas systems for DNA editing are widely employed, post-transcriptional manipulation of RNA remains difficult despite the prevalence of Cas9. Type VI CRISPR/Cas systems, which were recently found, allow for precise RNA editing without permanently affecting the genome. Cas13d has been put to good use in RNA-related studies across a wide range of RNA knock-down, and RNA detection without affecting DNA. Regulation of cas13d specificity and activity helps to avoid the off-target effects and immune responses in plants. Cas13d as highly efficient RNA-targeting tools for the virus resistance, gene function studies, disease diagnostics, and crop improvement in plants. However, CRISPR/Cas13d applications in plant RNA biology are just getting started. This article discusses how RNA editing tools derived from the CRISPR/Cas13d system are currently being used and where they may be used in the future for plant research.
CRISPR
CAS13D
PLANT BIOLOGY
RNA EDITING