Evidence for the presence of glutamate synthase in extracts of carrot cell cultures
Evidence for the presence of glutamate synthase in extracts of carrot cell cultures
- Biochem Biophys Res Commun 58:639-646, 1974 .
Artículo
Extracts of wild carrot, either dialysed or ~treated,i catalyse the oxidation of NADPH in the presence of a-ketoglutarate and L-glutamine. The oxidation does not Occur with ammonia at similar concentration to L-glutamine. NADH and L-asparagine can substitute in the reaction. Glutamine synthetase is also present in these extracts. The possibility that ammonia is assimilated in plants via glutamine synthetase and glutamate synthase now exists and is discussed.
Artículo
Extracts of wild carrot, either dialysed or ~treated,i catalyse the oxidation of NADPH in the presence of a-ketoglutarate and L-glutamine. The oxidation does not Occur with ammonia at similar concentration to L-glutamine. NADH and L-asparagine can substitute in the reaction. Glutamine synthetase is also present in these extracts. The possibility that ammonia is assimilated in plants via glutamine synthetase and glutamate synthase now exists and is discussed.