Differential effects of spermine on phosphatidylinositol 3-kinase and phosphatidylinositol phosphate 5-kinase (Record no. 39173)

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control field MX-MdCICY
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control field 20250625122447.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-4812
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245 10 - TITLE STATEMENT
Title Differential effects of spermine on phosphatidylinositol 3-kinase and phosphatidylinositol phosphate 5-kinase
490 0# - SERIES STATEMENT
Volume/sequential designation Life Science , 57(7), p.685-694, 1995
520 3# - SUMMARY, ETC.
Summary, etc. The metabolism of phosphoinositides plays an important role in the signal transduction pathways. We report here that naturally occurring polyamines affect the activities of phosphatidylinositol (PI)3-kinase and PI 4-phosphate (PIP)5-kinase differently. While polyamines inhibited the PI 3-kinase activity, they stimulated the activity of PIP 5-kinase in the order of spermine > spermidine > putrescine. Spermine inhibited the PI 3-kinase activity in a concentration-dependent manner with an IC50 of 100 microM. On the other hand, spermine (5 mM)stimulated the activity of PIP 5-kinase 2-3 fold. Kinetic studies of spermine-mediated inhibition of PI 3-kinase revealed that it was noncompetitive with respect to ATP. The effect of Mg2+ and PIP2 concentration on kinase activity was sigmoidal, with spermine inhibiting PI 3-kinase activity at all PIP2 concentrations. While 1 mM calcium stimulated PI 3-kinase activity at submaximal concentrations of Mg2+ (1.25 mM), inhibition was observed at optimal concentration of Mg2+ (2 mM). We propose that spermine may modulate the cellular signal by virtue of its differential effects on phosphoinositide kinases.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Singh, S.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ItngNQPrVNi9ZqSJBJUjntVJa0mcOf0V/view?usp=drivesdk">https://drive.google.com/file/d/1ItngNQPrVNi9ZqSJBJUjntVJa0mcOf0V/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-4812 25.06.2025 25.06.2025 Documentos solicitados