A new method for isolation of purified genomic DNA from haemosporidian parasites inhabiting nucleated red blood cells (Record no. 39178)
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| fixed length control field | 02006nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625122447.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-4817 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A new method for isolation of purified genomic DNA from haemosporidian parasites inhabiting nucleated red blood cells |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Experimental Parasitology, 133(3), p.275-280, 2013 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | During the last 10 years, whole genomes have been sequenced from an increasing number of organisms. However, there is still no data on complete genomes of avian and lizard Plasmodium spp. or other haemosporidian parasites. In contrast to mammals, bird and reptile red blood cells have nuclei and thus blood of these vertebrates contains high amount of host DNA; that complicates preparation of purified template DNA from haemosporidian parasites, which has been the main obstacle for genomic studies of these parasites. In the present study we describe a method that generates large amount of purified avian haemosporidian DNA. The method is based on a unique biological feature of haemosporidian parasites, namely that mature gametocytes in blood can be induced to exflagellate in vitro. This results in the development of numerous microgametes, which can be separated from host blood cells by simple centrifugation. Our results reveal that this straight forward method provides opportunities to collect pure parasite DNA material, which can be used as a template for various genetic analyses including whole genome sequencing of haemosporidians infecting birds and lizards. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Palinauskas, V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Krizanauskiene, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Iezhova, T. A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bolshakov, C. V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jönsson, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bensch, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Valkiunas, G |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1DIocLTmBET63FdgJHqMz20QP71MPkMUt/view?usp=drivesdk">https://drive.google.com/file/d/1DIocLTmBET63FdgJHqMz20QP71MPkMUt/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-4817 | 25.06.2025 | 25.06.2025 | Documentos solicitados |