Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot (Record no. 39203)

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control field 20250625122448.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-4842
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245 10 - TITLE STATEMENT
Title Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot
490 0# - SERIES STATEMENT
Volume/sequential designation Physiologia Plantarum, 111(3), p.389-395, 2001
520 3# - SUMMARY, ETC.
Summary, etc. Carrot (Daucus carota L. F1 hybrid Starca)excised hypocotyls were cultured on Murashige and Skoog medium with and without 2,4-dichlorophenoxy acetic acid (2,4-D)to determine the effect of this plant growth regulator on their further development and their endogenous hormone levels. Culture in the absence of 2,4-D stimulated root development at one end of the hypocotyl segments and increased the endogenous levels of free indole-3-acetic acid (IAA), zeatin/zeatin riboside and N6(Delta2-isopentenyl)adenine/N6(Delta2-isopentenyl)adenosine, as determined by radio-immunoassay. On the other hand, the presence of 2,4-D in the culture medium promoted callus induction and proliferation, together with abscisic acid (ABA)accumulation, in the hypocotyl segments during the first weeks of culture. When the callus segments generated in the hypocotyl sections cultured in the presence of 2,4-D were cultivated further, the development of two callus types was observed, one composed of preglobular and globular embryos and the other translucent, watery and lacking any sign of organisation. The embryos of the first type germinated when callus segments were transferred to regeneration conditions, while no change was observed when the second type was induced to regenerate. Higher levels of free IAA and ABA were obtained in the embryogenic calli when compared to the non-embryogenic, while no differences were observed among callus types in the other hormones evaluated. The possible role of the different plant hormones during induction of somatic embryogenesis is discussed.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Jiménez, V.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bangerth, F.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1OhD9_ySMsRy4O7Y4hi5sQ0rnQvf3dtl7/view?usp=drivesdk">https://drive.google.com/file/d/1OhD9_ySMsRy4O7Y4hi5sQ0rnQvf3dtl7/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-4842 25.06.2025 25.06.2025 Documentos solicitados