Molecular Karyotype of the phytopathogenic fungus sclerotinia sclerotiorum (Record no. 39238)

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control field MX-MdCICY
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control field 20250625122448.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-4877
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Title Molecular Karyotype of the phytopathogenic fungus sclerotinia sclerotiorum
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Volume/sequential designation Current Genetics, 29, p.496-501, 1996
520 3# - SUMMARY, ETC.
Summary, etc. Molecular techniques have been used to characterize different field isolates of Sclerotinia sclerotiorum, an ubiquitous phytopathogen. Chromosomal DNA resolved by pulsed-field gel electrophoresis (PFGE)revealed that S. sclerotiorum contains at least 16 chromosomes ranging from 1.5 Mb to 4.0 Mb. The size of the haploid genome was estimated to be 43.5 Mb. Six field isolates with different levels of virulence on sunflower germlings or green beans were differentiated by random amplification of polymorphic DNA (RAPD), and analysed by clamped homogeneous electric field electrophoresis. This analysis revealed few chromosome-length polymorphisms among these strains. Chromosomal DNA hybridization indicated that the endopolygalacturonase-encoding pg1 gene is localized on the smallest chromosome of all the strains, whereas the ribosomal DNA mapped to different-sized chromosomes. The less-aggressive strain was characterized by the presence of a supernumary small band, presumably consisting of dsRNA. In contrast to numerous other phytopathogenic fungi, this study reveals a strong karyotypic stability among the strains of S. sclerotiorum which may be preserved by the sexual mode of reproduction of this species
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Fraissinet-Tachet, L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Reymond, P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Févre, M.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1n3seVKldRvARToH2k7OTz7JVR2X9YUcc/view?usp=drivesdk">https://drive.google.com/file/d/1n3seVKldRvARToH2k7OTz7JVR2X9YUcc/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-4877 25.06.2025 25.06.2025 Documentos solicitados